This volume is the proceedings of ECAL 2017, the Fourteenth European Conference on Artificial Life, held September 4–8th 2017, in Lyon, France (https://project.inria.fr/ecal2017/). Since the first ECAL in 1991, the conference is the main international event of the International Society for Artificial Life in odd-numbered years, alternating with ALife, the International Conference on the Synthesis and Simulation of Living Systems. The theme of this edition of ECAL was “Create, play, experiment, discover: The experimental power of virtual worlds”. The volume contains the abstracts of the seven invited presentations, as well as 87 contributed articles selected by the programme committee based on at least three independent reviews. Contributions are either long (up to 8 pages) or short (up to 2 pages) articles. Long articles present original results, while short articles are extended abstracts presenting either original work or recently published work. These contributions cover all the topics of artificial life, including: artificial chemistry; origins of life; self-replication, self-repair and morphogenesis; evolutionary dynamics; ecological dynamics; social dynamics; computational cellular biology; computational physiology; bio-inspired robotics; evolutionary robotics; perception, cognition and behavior; evolution of language and computational linguistics; embodied and interactive systems; collective dynamics of swarms; complex dynamical systems and networks; cellular automata and discrete dynamical systems; economic and social systems as living systems; computational humanities; methodologies and tools for artificial life; interactions between in silico/in vitro/in vivo experiments; philosophical, epistemological and ethical issues; artificial life and education; artificial life-based art; applications of artificial life; living technologies.